Effect of different concentrations of sucrose, glucose and fructose on microtuberization of sweet potato (Ipomoea Batatas)
Date
2012-06
Journal Title
Journal ISSN
Volume Title
Publisher
U. P
Abstract
The effect of different concentrations of sucrose, glucose and fructose on in vitro microtuberization of sweet potato (Iponwea Nitwits) was studied. Plantlets were obtained from explants excised from mother tubers of sweet potato and cultured on Murashige and Stoog (MS) media without growth regulators. Growth characteristics after three weeks revealed that plantlets developed roots within 12 hours with lengths of 20-38cm; and developed shoots within 3-5 days with plantlet heights of 12-16. 5cm. Also, the number of leaves per plantlet was 5-20 and the nodal number was 12-1.4 per plantlet. Microtubers were formed from plantlets cultured on 3%, 5% and 8% concentrations of the three sugars as basic. carbon sources; with 5% and 8% concentrations yielding the biggest sizes of microtubers. This implies that concentrations of sucrose, glucose and fructose ranging between 3% and 8% are capable of forming sweet potato microtubers in vitro, hence facilitating mass production of microtubers which can be supplied to farmers at subsidized rate
Description
The article contains tables
Keywords
Concentrations, sucrose, glucose, fructose, sweet potatoes, microtuberization, Department of Biotechnology
Citation
Okereke, J. N., Nnoli, M. C. & Okechukwu, R. I. (2012). Effect of different concentrations of sucrose, glucose and fructose on microtuberization of sweet potato (Ipomoea Batatas). International Journal of Environmental Health and Human Development, 13(1), 59 - 64