School of Biological Sciences

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Open Access
Bioaccumulation of lead by Bacillus species isolated from pig waste
(Ficus Publishers, 2012-02-07) Akujobi, C. O.; Odu, N. N.; Okorondu, S. I.
Heavy metals, such as lead, copper, cadmium, chromium and mercury are important environmental pollutants, particularly in areas with high anthropogenic pressure. Their presence in the atmosphere, soil and water, even in traces, can cause serious problems to all organisms. Living organisms are exposed in nature to lead commonly in their ionized forms, which at different concentrations affect microbial population. Microorganisms are known to interact with heavy metals through a number of mechanisms including intracellular accumulation. Bacillus species isolated from pig waste was exposed to different concentrations of lead solution within 24 hours. The percentage log survival / growth rate in the different concentrations of lead was determined periodically. Bioaccumulation of lead by the test isolate was determined in the graded lead concentrations (0, 1.10, 100. 500 µg/ml). The result showed that the growth of the isolate was progressively inhibited by lead in a dose dependent fashion. The isolate showed a potential to survive lead intoxication and accumulated the toxicant. Therefore, Bacillus species isolated from pig waste shows a promise for its use in bioremediation of lead polluted environments. This can be applied as organic manure together with the microorganism in heavy metal-polluted site to prevent heavy metal toxicity and to enhance the growth of plants.
Open Access
Asymptomatic bacteriuria in pregnant women at the outpatient clinic of some governmental hospitals in Imo State, Nigeria
(International Formulae Group, 2009) Akujobi, C. O.; Ogbulie, J. N.; Umeh, S. I.; Abanno, N. U.; Nwachukwu, I. N.
Asymptomatic bacteriuria among pregnant women in Imo State, Nigeria was studied. All pregnant women attending antenatal at Federal Medical Center, Owerri, General Hospital, Okigwe, and Imo State University Teaching Hospital, Orlu, between January and April 2006 and who agreed to enter the study were clinically evaluated to exclude signs of urinary tract infection. Samples of 10-15 ml urine were examined for bacteria, pus cells and parasitic ova. The samples were further cultured on cysteine lactose electrolyte deficient agar and colony counts yielding bacterial growth of 105 or more of pure isolates were deemed significant. Of the 630 pregnant women, 111 had significant bacteriuria giving a prevalence rate of 17.6%. The highest age specific prevalence was found in the 31-40 year-olds and the lowest in the 41-50 year-olds. Socioeconomic status of the respondents had effect on the prevalence of bacteriuria and also, there was an increase in the prevalence with increase in age of gestation. The dominant isolates were Staphylococcus species and Klebsiella species with most of the isolates obtained in respondents from Owerri and Okigwe study areas.
Open Access
Antibiotic-resistant Escherichia coli in a government piggery farm in Owerri, Nigeria
(International Formulae Group, 2008) Akujobi, C. O.; Ogbulie, J. N.; Umeh, S. I.; Abanno, N. U.
The acquisition of antibiotic-resistant commensal Escherichia coli was examined in a cohort of newborn pigs. Faecal samples were collected weekly from young pigs over a 2 month period and screened for E. coli resistant to ampicillin, nalidixic acid, gentamicin and ciprofloxacin at concentrations of 16, 8, 8 and 8mg/l, respectively. E. coli viable counts were also performed on the samples. All the pigs acquired gentamicin-nalidixic acid- and ampicillin-resistant E. coli, while 80% acquired ciprofloxacin-resistant E. coli during the study. Sixty-five per cent of samples were resistant to at least one of the four antibiotics. The prevalence of the antibiotics declined significantly with age and time (p<0.05). Total counts and antibiotic resistant counts declined with age (p<0.05), with the rate of decline in antibiotic resistant counts being greater than that for total counts (p<0.05). The cohort pigs rapidly acquired antibiotic-resistant bacteria shortly after birth and the carriage of resistant bacteria was associated with age.
Open Access
Extraction of bioethanol from pretreated sawdust
(U. P., 2020-06) Ajah, Chinonye Peace; Akujobi, Campbell; Wesley, Braide
In this study, optimization of brix conversion of wood shavings by Saccharomyces cerevisiae was investigated. Wood shavings were collected from timber shade at Naze, Owerri Imo state. Chemical pretreatment and hydrolysis was evaluated. Brewer’s yeast (Saccharomyces cerevisiae) was used as the isolate for fermentation. A five factor box behnken design was used. The effect of incubation temperature(30-40oC), inoculum size(0.85),incubation time (72-120hours), wood concentration( 10-20g), and NaOH concentration (3.5-4.5%) were studied using response surface methodology (RSM). Brix concentration was used as a response in the resulting experimental design. The statistical analysis of the constructed model developed by RSM suggested that linear effects of NaOH concentration and wood concentration were both significant (p<0.05) for biomass converted. The optimum conditions for the conversion of brix are wood concentration of 15.66g, NaOH concentration of 4.47%, inoculum size of 0.85, incubation time of 72hours and incubation temperature of 40oC. Ethanol yield obtained was 1.68-2.25%. At these conditions, the isolate used was able to convert 60.97% of the brix in the wood extract to ethanol.
Open Access
Parametric optimization of synthesis of silver nanoparticles from Mangifera indica and Prunus dulcis extracts and their antibacterial activity
(University of Oradea Publishing House, 2020) Akujobi, Campbell Onyeka; Anuforo, Henry Uzoma; Okereke, Josephat Nwabueze; Ibeh, Chinyere; Agbo, Chioma Joy
Optimization of process parameters is crucial to the deployment of nanotechnology as a competitive source of novel materials to many fields. The present study has lent credence to the simplicity, environmental friendly, cost effective and quick potentials of using plant extracts as source of capping and stabilization agents in the synthesis of silver nanoparticles (AgNPs). Aqueous leaf extracts of Mangifera indica and Prunus dulcis were used in synthesis of silver nanoparticles. The study was designed with Box Behnken Design (Minitab® 17) to optimize temperature (25 – 35°C), pH (6 – 8) and time of reaction (6 – 24hours). Fifteen runs were obtained for each sample which determined the value of each parameter used for the synthesis. Results obtained were subjected to Response Optimizer (Minitab® 17) which predicted optimum conditions for synthesis of silver nanoparticles as 25°C at pH 8 and 10.24 hours with predicted maximum yield of 2.53 for Prunus dulcis. However, the actual yield of silver nanoparticles under these conditions was 2.64. For Mangifera indica leaf extract, the predicted optimum conditions were 31.4°C at pH of 8.0 and 9.39 hours with predicted maximum yield of 2.55. Nevertheless, the actual yield under the optimum conditions was 2.61. Results show that Prunus dulcis extract has relatively higher potential yield for silver nanoparticles than Mangifera indica extracts. UV-Vis spectrophotometer showed that the absorbance for synthesized silver nanoparticles using both plant extracts peaked between 400 – 430nm. Silver nanoparticles from both plants showed activity against Bacillus subtilis and Pseudomonas aeruginosa, though B. subtilis was more sensitive. However, silver nanoparticle from Prunus dulcis was less effective against the bacteria.
Open Access
Level of heavy metals in soil samples from farmlands along highways in parts of Owerri, Nigeria
(U. P., 2019-07) Okereke, J. N.; Nduka, J. N.; Ukaoma, A. A.; Ogidi, I. O.
The research was designed to ascertain the concentrations of heavy metals (Cu, Pb, Zn, Ni, Cd) in soil samples from farmlands along highways in parts of Owerri. Soil samples were collected from cassava farms along three major roads with high traffic density and a rural road that served as control location, for possible heavy metals contamination due to vehicular emission. Standard laboratory procedures were adopted to determine the concentration of heavy metals using Atomic Absorption Spectrophotometer (AAS). Contamination factor (CF) and Pollution Load Index were mathematically calculated. The results revealed that the mean concentrations of metals in soil samples in mg/kg ranged from 3.00 – 6.31 for Cu; Pb, 0.09 – 4.00; Zn, 3.97 – 8.59; Ni, 0.001 – 0.96; and Cd, 0.31 – 0.79. These metals showed decrease in concentrations with increase in distance away from the highway. The values of contamination factor (CF) were low except for Cd and Pollution Load Index (PLI) for all soil studied showed no sign of pollution. There was no correlation between traffic density and concentration of heavy metal in soil except for Cd (Rs= 0.800). The overall results showed evidence of some heavy metal pollution on the soils with possible cumulative effect over time.
Open Access
Analysis of heavy metals concentration in prawn (Macrobrachium Sp) harveted from Gberikoko River, in Sapele Delta State, Nigeria
(U. P., 2015-08) Obasi, kalu O.; Enemchukwu, S.; Okereke, J. N.; Ezeji, E. U.; Oparaigbo, M. I.
The concentrations of selected heavy metals: cadmium, arsenic, lead and mercury in prawn (Macrobrachium sp) were analyzed. The morphometric records of the prawn samples were taken. The specimens were subsequently weighed and individually pulverised to a uniform particle size. Ten(10)grams of each pulverised sample were put in a 200ml digestion conical flask, 30ml of aqua regia, which constitutes of hydrochloric acid, nitric acid and hydroflouric acid in the ratio 1:3:1 was added and placed uprightly for 10-15 minutes. The mixture was heated in a water bath until the whole sample dissolved and a yellow tint of the solution appeared. The solution was filtered using a filter paper into centrifuge tubes and made up to 50ml mark with deionized water. It was transferred into sample vials for analysis. The heavy metal analysis was done according to the standard methods for heavy metal determination using the FS 240 Varian Atomic Absorption Spectrophotometer. Lead had the highest concentration in sample F and the lowest in sample H, with concentrations of 1.75ppm and 1.54ppm respectively. Also, the prawn samples exhibited the highest record of Mercury and lowest mean value of Arsenic with values of 6.9±3.95and0.00±0.00 respectively. For Mercury, the highest concentrations occurred in sample G and lowest in F, with concentrations of 12.6ppm and 4.1ppm respectively. Arsenic showed its highest concentration in sample F and lowest in sample H with concentrations of 0.65ppm and 0.64ppm, respectively, whereas Cadmium wasn’t detected in any of the samples. From the analysis of data, since F > Fcrit and p < 0.05, there is a significant difference in the mean concentrations of the prawn samples from Gberikoko river in Sapele, Delta State Nigeria.
Open Access
Bacteriological quality of water contaminated with faecal wastes from livestock
(Science Publishing Group, 2015) Anyadoh-Nwadike, S. O.; Okereke, J. N.; Odah, R.; Obijekwu, O.; Okorondu, S. I.; Obasi, K. O.
Water is very important to life but when contaminated, it can be deleterious to life. Livestock from various farms produce large amount of faecal wastes. Most cattle farmers move their animals from place to place around Imo State, Nigeria, in search of pasture, these animals defeacate indiscriminately. Again, many farmers indulge in indiscriminate disposal of animal wastes. These faecal wastes can be carried by runoff water into surrounding water sources causing them to become contaminated. This study was therefore carried out to determine the prevalence of possible pathogenic bacteria in water contaminated with poultry litters, cow and pig dungs. Faecal samples from poultry, cows and pigs were collected from School of Agriculture and Agricultural Technology (SAAT) farm in the Federal University of Technology Owerri (FUTO), Imo State, Nigeria. The contaminated water samples were examined for the prevalence of bacteria. Isolation and characterization of bacteria were done using standard microbiological protocols. Results revealed the presence of Escherichia coli, Enterobacter aerogenes, Serratia marcescens, Erwinia amylovora, Vibrio cholerae, Pseudomonas aeruginosa, Citrobacter freundii, Proteus vulgaris, Staphylococcus aureus, Bacillus subtilis, Streptococcus faecalis and Salmonella typhi. Pseudomonas aeruginosa was the most prevalent isolate followed by Escherichia coli and Vibrio cholerae. The presence of these organisms of public health importance in water contaminated by livestock faeces calls for continuous surveillance of various water sources prone to faecal contamination. Appropriate treatment and disposal methods of these faecal samples as well as appropriate treatment of water source prone to such contaminants should be adopted so as to avert the possible health hazards resulting from use of water from such contaminated sources.
Open Access
Biosorption of Cd and Pb in textile effluent using musasapientum (Banana) peels
(U. P., 2016-06) Okereke, J. N.; Ogidi, O. I.; Obasi, K. O.
Abstract: The efficacy of using Musasapientum (banana) peels was tested for the removal of Cd and Pbusing batch experiments from textile industrial effluent. Standard spectophotometric techniques using AAS were adopted to assess the concentrations of the heavy metals before and after treatment of effluent with the biosorbent (banana peels). Metal sorption increased proportionally with the equilibrium metal concentration. The adsorption capacity of banana peels on the metal ions was of the order: Cd >Pb, with percentage removal of metal ions as 99.93% for Cd and 99.64% for Pb. Biosorption equilibrium isotherms were plotted for metal uptake capacity (q) against residual metal concentration (Cf) in the sample and mathematically expressed by Langmuir and Freundlich models. The values of separation factor were between zero and one, indicating favorable sorption for the two metals (Cd and Pb) on the biosorbent. Peels of M. sapientum showed comparable biosorption capacity for Cadmium and Lead with other types of biosorbent materials found in literature and are cost effective, economical and eco-friendly in removal of these heavy metals from textile effluent sample.
Open Access
Efficiency of water-purifying devices used in homes and industries
(U. P., 2013-12-28) Okereke, J. N.; Nnoli, M. C.; Okereke, C. B. N.; Ahumibe, N. C.
The use of water-purifying devices are gaining popularity, as many homes and industries are using them to treat water for dinking. However, most of the users do not know much about the performance/efficiency of these devices, hence this investigation. Efficiency of water-purifying devices were evaluated to determine their efficiencies in treating water consumed by people. Raw water samples from groundwater source, borehole (a major source of drinking water) in Owerri, Nigeria were treated using commonly used water treatment devices: resin ion-exchanger, sand-bed filter, activated carbon filter, micron filter, reverse osmosis membrane filter, ozonator, and UV-sterilizer. The resulting purified water samples were labeled, and each were subjected to physical, chemical and bacteriological analyses using APHA (2006) water analysis method. Raw water (control) sample had pH value of 6.7 while the pH values of treated water samples fell within 6.9 – 7.1. Turbidity value of control sample was 6.0 NTU with other samples recording 4.0 NTU and below. Total heterotophic bacteria count and total coliforms counts of the raw water sample were 4.0 x 102 CFU/ml and 2.2 x 102 CFU/100ml respectively, but were below 2.5 x 102 CFU/ml and 1.9 x 102 CFU/100ml respectively in some of the treated samples, and nil in others. The overall efficiency of the treatment devices were of the order: Reverse osmosis membrane filter (76.25%) > UV-sterilizer (72.24%) > micron filter (65.85%) > Ozonator (62.97%) > Activated carbon filter (51.86%) > Resin ion-exchanger (46.67%) > Sand-bed filter (43.55%). This implies that not all water treatment devices used in homes and indutries are efficient enough to yield qualitative drinking water for unsuspecting users.
Open Access
Effect of dichlorvos on reproductive performance of laying hens
(Academic Journals, 2016-04) Ezeji, Ethelbert Uchechukwu; Udebuani, Angela Chika; Okereke, Josephath; Anyadoh-Nwadike, Sylvia; Onwurah, Ikechukwu. N. E.; Obasi, Kalu
The effect of dichlorvos on reproductive performance of laying hens was studied. Seven weeks old pullets with an average weight of 557.5 ± 9.5 g were divided into four groups and fed ad libitum with commercial poultry feeds contaminated with 0.01, 0.02 and 0.04% dichlorvos (w/v). The group without dichlorvos served as the control. Exposure to dichlorvos was continued until nine weeks after the hens started laying eggs. There was a significant reduction (p<0.05) in feed intake between the control group and those exposed to dichlorvos. Egg laying was delayed in the hens exposed to the pesticide by as much as eighteen weeks. The ages of the hens at first egg lay were 18 weeks for the control, 23 weeks for hens fed on 0.01 and 0.02% contaminated diet and 36 weeks for those fed on 0.04% contaminated diet. The average daily egg production was reduced from 5 eggs in the control group to 1 egg in 0.04% contaminated group. The protein contents of the egg (yolk and egg-white) and cholesterol level of the egg yolk were lowered in birds exposed to dichlorvos. There was no significant difference in the weight of eggs between the control and those exposed to pesticide. Results of this study suggest that exposure of laying hens to dichlorvos could affect their reproductive success.
Open Access
Effect of dichlorvos on the fertility of adult male albino rats
(Science Publication, 2015) Ezeji, Ethelbert Uchechukwu; Ogueri, Oluchi Dominica; Udebuani, Angela Chika; Okereke, Josephath N; Obasi, Kalu O
Continuous use of pesticides could have adverse effects on the reproductive functions of humans and non-target organisms. This study was carried out to examine the effect of dichlorvos on the reproductive parameters of adult male albino rats. Twenty (20) adult male Wistar albino rats weighing between 130 – 225g were divided into 4 groups of 5 animals each. Three groups were given water contaminated with 0.01, 0.03 and 0.05% dichlorvos (DDVP) respectively. The control group was given distilled water without dichlorvos. All the animals were maintained on commercial animal feed (growers mash) throughout the period of the experiment which lasted for 28 days. There were significant reductions (p<0.05) in testosterone levels in rats exposed to dichlorvos. Testicular weight (testis and epididymis) and sperm count were significantly lowered in rats exposed to dichlorvos. Histological examination of the testes revealed levels of distortions in the cells of the seminiferous tubules as well as hypertrophy of the spermatogonia cells. These results show that dichlorvos has adverse effects in the reproductive functions of male albino rats.
Open Access
Effects of anthropogenic activities on soil carbon storage and compactness in coastal plain soil of tropical urban area
(U. P., 2015-11) Udebuani, A. C.; Obasi, K. O.; Ezeji, E. U.; Okereke, J. N.; Anyado, S. O.; Dike-Ndudim, J. N.; Enekwu, E. C.
The study on the impact of anthropogenic activities of a tropical urban soil; effects of different land use on bulk density, relative bulk density and their capacity to store carbon were investigated using several key soil physical and chemical properties such as ammonium acetate methods used for determination of cation exchange capacity. Results obtained showed the soil textural class to be sandy in all the land used area. Moisture content and porosity were highest in the automobile servicing area. Bulk density (1.50g/cm3) and relative bulk density (0.82g/cm3) were highest in soil samples from commercial area. Automobile servicing area had the lowest soil pH (5.1), compared to other land use areas, which had it pH ranging from 6.2 – 7.5. The highest value of exchangeable base was obtained also from automobile servicing area compared to other anthropogenic active areas. There is a considerable variation in the total carbon storage from different land uses with the highest carbon storage obtained from automobile servicing area (570.5 g/cm3), this is followed by soil samples from commercial areas (370.0 g/cm3). The lowest value of carbon storage was obtained from institutional area. The major effect of anthropogenic activity is on soil organic matter (OM); therefore, the degree of compactness was as a result of the impact on organic matter. Result of this present investigation shows that anthropogenic activities in urban environment affect the compactness and carbon storage of soil.
Open Access
Physico-chemical and bacteriological assessement of Oyibo River in Ehime Mbano, Imo State
(U. P., 2015) Okereke, J. N.; Okanume, J. N.; Okereke, C. B. N.
The physico-chemical and bacteriological assessement of Oyibo river, Ehime Mbano, was carried out between 2011 and 2012. Considering the communities that make use of this river for different purposes, water samples were collected from four different sampling points along the river and subjected to physico-chemical and bacteriological analyses. Pour plate method was adopted to determine total heterotrophic bacteria, and membrane filtration technique, for total coliforms and E. coli counts. Standard procedures for water analysis by APHA were adopted for the determination of physical and chemical parameters. Atomic Absorption Spectrophptometer (AAS) was used to analyze for heavy metals. The average values of total heterotrophic bacteria were 5.8 x 103 cfu/ml and 1.51 x 103 cfu/ml for rainy and dry seasons respectively. Average values for total coliforms and E. coli counts were 33.75 cfu/100ml and 6 cfu/100ml for rainy season, and 17.38 cfu/100ml and 4 cfu/100ml for dry season. Bacterial isolates included E. coli, Salmonella spp, Lactobacilli spp, Klebsiella spp, Staphylococcus spp, Proteus spp, and Pseudomonas spp. In all the water samples, the pH values ranged from 6.04– 6.52. Average values of total hardness for rainy and dry seasons were 5.90mg/l and 5. 43mg/l respectively. The concentrations of nitrate, phosphate, chloride, and sulphate ranged from 0.00 16.40mg/l; 0.01– 1.24mg/l; 0.13– 0.35mg/l; 0.00– 15.95mg/l respectively. Average COD, BOD and DO values were 5.63mg/l, 2.07mg/l, and 3.17mg/l in dry season, and 9.24mg/l, 1.77mg/l, and 4.26mg/l in rainy season. The values of heavy metals during the rains were within the range of 0.0008ppm– 1.5605ppm, but 0.0001ppm– 0.0339ppm, in dry season. There were no significant differences in some bacteriological and chemical properties with season, but the physical parameters such as depth, flow rate, pH, and turbidity were, at p ˂ 0.05. The study revealed that Oyibo River is not safe for drinking (inasmuch as it serves as source of drinking water for some communities), unless subjected to appropriate treatment.
Open Access
Possible health implications associated with cosmetics: A review
(Science Publishing Group, 2015) Okereke, J. N.; Udebuani, A. C.; Ezeji, E. U.; Obasi, K. O.; Nnoli, M. C.
Cosmetics are products applied to the body or face to improve appearance. They cleanse, beautify, promote attractiveness and alter the appearance of the body. The concept of beauty and cosmetics is as old as mankind and civilization. Assorted beauty products such as skincare products, hair products, fragrances, oral hygiene, and nail products, which may contain toxic chemicals that can be harmful to health are used especially by women. Most cosmetic products contain hazardous chemicals like Sodium Laureth Sulphate, Talcum, Parabens, Coal tar dye, Phthalates, Fragrance, Mineral oil, Triethanolamine and some heavy metals eg, Lead, Arsenic, Nickel, Cadmium, and Mercury. The bioaccumulation of these harmful chemicals and metals in the body over time has been associated with cancer, reproductive and developmental disorders, contact dermatitis, hair loss, lung damage, ageing, skin disease and reaction, allergies, and damage of nails. The deleterious chemicals and metals can enter into the body by inhalation of perfumes, deodorant, nail polish, scented powder, etc; by absorption through penetration of harmful chemicals from body creams, moisturizers, cleanser, eye shadow, etc. It can also be by ingestion of chemicals and metals in lip sticks, lip balm, lip gloss, etc. through the mouth.
Open Access
Environmental and health impact of industrial wastewater effluents in Nigeria - A review
(U. P., 2016) Okereke, J. N; Ogodi, O. I.; Obasi, K. O.
Wastewater effluents from industries particularly in developing countries like Nigeria are in most cases discharged into the adjoining environment; water bodies being mostly affected. Some of these wastewater effluents are untreated or inadequately treated before being discharged, which has become a worrisome phenomenon due to its impact on environmental health and safety. This paper is aimed at reviewing the environmental and health impacts of untreated or inadequately treated industrial wastewater effluents. The quality of wastewater effluents is responsible for the degradation of the receiving water bodies. This is because untreated or inadequately treated wastewater effluents may lead to eutrophication of the receiving water bodies and also create environmental conditions that favor proliferation of water-borne pathogens or toxin-producing cyanobacteria. In extension, recreational water users coming into contact with the infected water are at risk. Although various microorganisms play many beneficial roles in wastewater systems, a great number of them are considered to be critical factors in contributing to numerous water-borne diseases outbreak. Also, wastewater effluents have been shown to contain a variety of anthropogenic compounds, many of which have endocrine-disrupting properties. Since large amounts of wastewater effluents are passed through sewage treatment systems on a daily basis, there is a need to remedy and diminish the overall impacts of these effluents in receiving water bodies. In order to comply with wastewater legislations and guidelines, there is a need for adequate treatment before discharge. This can be achieved through the application of appropriate treatment processes, which will help to minimize the risks to public health and the environment. To achieve reduced discharge of wastewater into receiving water bodies, careful planning, adequate and suitable treatment, regular monitoring and appropriate legislations are necessary.
Open Access
Environmental impact of palm oil mill effluent and its management through biotechnological approaches
(U. P., 2020) Okereke, J. N.; Ginikanwa, R. C.
Globally, palm oil production is on the increase and the pollution caused by waste materials from the palm oil mills has become a major environmental concern. However, not much has been said about the negative effects of such a huge industry. This report reviews the environmental impacts of palm oil mill effluents generated from oil palm processing and how they can be efficiently managed through biotechnological tools. The processing of oil palm generates three (3) major waste streams including gaseous (pollutant gases), liquid (palm oil mill effluent, POME) and solid (palm press fibre, chaff, palm kernel shell and empty fruit bunch) wastes. POME has been the most problematic environmentally, among all the others. It contains high pollution indicators such as oil and grease; it also impacts on the soil and water properties. Discharging POME into the soil tampers withits pH, which is one of the major factors affecting nutrient availability to plants. POME also changes the soil appearance and properties in terms of vegetation, odour, colour and constitution, making the soil to lose its vegetative cover. This leads to water logging of the pores and subsequent death of vegetation, leaving the environment bare. During the rainy season, POME serves a breeding habitat for mosquitoes and produces offensive odours. When discharged into rivers, streams or surrounding lakes, the water turns brown, smelly and slimy, aquatic organisms get killed, and local people are denied the availability of water for domestic uses and fishing. The discharge of POME on the soil and surrounding lands can also contaminate the aquatic ecosystem during runoff, leading to acidification and eutrophication. The paper concludes by suggesting options for effective management such as the use of POME for microalgae cultivation, the use of pre-treated POME as fertilizer; focusing on phytoremediation, bioremediation and mycoremediation of POME-contaminated soil and water as options for the rehabilitation of POME-contaminated soils and water bodies.
Open Access
Genotoxicity of hairdressing salon effluent using allium cepa
(U. P., 2020) Okereke, J. N.; Dike, Ijere. N.; Ukaoma, A. A.; Ezejiofor, T. I. N.
Genotoxicity of hairdressing salon effluent using Allium cepa was carried out using different concentrations of 12.5%, 25%, 50% and 100% of hairdressing salon effluent obtain from different outlets. The presence and concentrations of some heavy metals such as lead, Mercury, Zinc, Cadmium and Arsenic in the effluent were determined using Atomic Absorption Spectrophotometer (AAS). Chromosomal aberration assays were used to determine the mitotic index and chromosome aberration rate. There was an increase in the mitotic inhibition as the concentration increased whereas there was a decrease in the mitotic index, indicative of an inverse relationship shared by the two properties. The effluent induced chromosomal aberrations in the meristematic cells of the A. cepa root tip, and laggards were the most frequently recorded aberrations induced. Morphological abnormalities were also recorded in the roots of the Allium cepa. The genotoxic effects of hairdressing salon effluent on Allium cepa indicates that it contains toxic substances which may constitute environmental risks particularly on soils and plant crops planted near hairdressing salon facilities.
Open Access
Geo-helminthes associated with geophagic pupils in selected primary schools in Oyi, Anambra State
(Science Publishing Group, 2015) Okereke, J. N.; Obasi, K. O.; Nwadike, P. O.; Ezeji, E. U.; Udebuani, A. C.
Identification of geo-helminthes associated with geophagy as well as risk factors predisposing to such infestations among primary school pupils were carried out. Ethical permissions were obtained from headteachers of schools and pupils to collect stool samples of 200 volunteer pupils, aged 5-13years (106 males and 94 females) from rural and urban primary schools in Oyi Local Government Area of Anambra State, Nigeria. Stool samples were examined microscopically using wet mount (normal saline and iodine) technique. Data collected via questionnaires were analyzed to assess the relationship between geophagia, regular ingestion of soil and soil-transmitted parasites. Three intestinal parasites: Ascaris lumbricoides (A.lumbricoides), Ancylostoma duodenale (A. duodenale) and Trichuris trichiura (T. trichiura) were commonly observed. A total of 178 pupils (89%) found to be infected with one or more of these worms. A.lumbricoides had the highest prevalence of 52.8%, while A.duodenale and T.trichiura had 32.58% and14.61% respectively in all five villages in the Local Government Area. Infection rates and geophagic habits were established to be highest in children between the ages of 5 and 7years (p>0.05). Location and sex were observed to be insignificant factors in the distribution of these parasites (p>0.05). Geophagy and any other form of contact with the topsoil and children orally should therefore be noted as an important risk factor for orally acquired parasitic infections among children. Education on geophagy prevention should be an integral component of all soil-transmitted parasite control programs.
Open Access
Performance of Zea Mays on soil contaminated with petroleum (Oily) sludge
(U. P., 2016) Okereke, J. N.; Ogidi, O. I.; Udebuani, A. C.; Onyekachi, U. C.; Ukaoma, A. A.; Obasi, K. O.
The study on the performance of Zea mays on soil contaminated with petroleum (oily) sludge was carried out using petroleum sludge sample from Kolocreek Rig in Port-Harcourt, Rivers State of Nigeria. Different concentrations of sludge contamination on soil were prepared and yellow and white maize seeds planted on them. Sludge and soil samples were subjected to laboratory analysis to determine their heavy metal concentrations (Nickel, Lead, Cadmium and Chromium) and total petroleum hydrocarbon (TPH) content, using Atomic Absorption Spectroscopy (AAS). Physical parameters of maize plants (plant height and average leaf width) were measured on weekly basis to determine the physical performance of the maize plant on petroleum sludge-contaminated soil using calibrated meter rule. Yellow and white maize seeds planted on uncontaminated soil (0% sludge) had 100% germination, seeds on 10% sludge had 75% and 50% germination respectively for yellow and white maize; seeds on 25% sludge had 25% germination each for the two maize types. Soil-plant transfer factor or bioaccumulation factor, f of the heavy metals by the plant showed that maize plant was able to take up the heavy metals from the soil (for example, f-value for Cr = 0.83 in 10% sludge, Ni = 0.91 in 25% sludge, Cd = 0.77 in 5% sludge, etc). Petroleum (oily) sludge contamination had significant effect on the general performance of Zea mays (p > 0.05). Maize plant has the ability to bioaccumulate (take-up) heavy metals and hydrocarbons from the soil thus possess high phytoremediation potential.