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Item Open Access Recombinant phosphoinositide-3-kinase C2β C2 domain molecules(IISTE, 2017) Banigo, Alma Tamunonengiofori; Azeez, Taofik OladimejiRecombinant DNA domain remains a dependant of effective use of recombinant proteins in many applications but its production remains an area of concern due to DNA vector and production path. This work is aimed at studying the generation and replication of the recombinant DNA molecule using PI3K C2β C2 domain and glutathione s-transferase (GST) which expressed in pGEX-2T vector. The molecular cloning technique was employed to generate a recombinant DNA molecule. PI3K C2β C2 domain of isoform PI3K C2β belonging to class PI3K C2 of Phosphoinositide-3-Kinase (PI3K) family was used and the effective digestion of pGEX-2T vector was studied using restricted enzymes (RE) of EcoR I and Sma I with binding partners location. The ligated product of recombinant DNA was obtained after successful doubled digestion of pGEX-2T vector. The best transformation of competent bacterial cells was obtained at molar ratio of 5:1 for RE digested vector insert DNA and vector DNA. The recombinant DNA can be employed may be used in treatment of non – communicable diseases such as cancer and diabetes.Item Open Access Soil quality modeling of a highly acidic eutric - tropofluvent soil(IDOSI Publications, 2010) Uzoije Atulegwu PatrickWaste from garri processing centre of Umuagwo, Ohaji L.G.A of Imo state has impacted enormous pollution on the soil ecosystem of the centre. Huge resources and quality man hour have been spent on determining the level of such pollution in similar circumstances using laboratory techniques. In view of the aforementioned, the study developed several regression models, establishing the relationship between Ph and other selected soil physiochemical properties, to predict the values of the these selected properties in the polluted soils. The study took place in three sampling pedons A, B and C using the technique of stratified random sapling for sample collection. Pedon A is the background or the control unit, pedon B or the discharge point is the cassava mill effluent (CME) receiving unit and pedon C is the down stream, 500m away from pedon B. the result showed high values of soil physiochemical properties(mainly the heavy metals) at pedon B and the trend reduced away from pedon B and lowest in pedon A. In terms of pedons the heavy metal concentrations took this trend; pedon B>pedon C>pedonA. pH relationship with heavy metals in pedons A and B showed positive linear relationships with their coefficient of linearity(R2) ranging between 0.70-0.92 while other soil physiochemical properties obeyed polynomial relationships at reasonably high coefficient of fitness. Polynomial models fitted the pH relationships virtually in all the selected physiochemical properties of pedon C. The models have provided quick assessment of these physiochemical materials by mere determining the pH value and substituting the pH values into the models to predicting other properties.