Impact of petroleum effluent from jetty sites on physicochemical properties and microbial profile of an agricuItural soil
Date
2023-08
Authors
Journal Title
Journal ISSN
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Publisher
Federal University of Technology, Owerri
Abstract
Petroleum effluents from oil producing companies have negatively affected all spheres of live including agricultural soil. In the study, soil samples were with 10%, 50% and 100% of the petroleum effluents from Onne, Nembe and Escravos. Soil physicochemical properties, soil stress marker enzymes, soil bacteria populations, emulsification properties of bacterial isolates and their molecular properties and spectro photometric assessment of their emulsion were studied using standard methods. Effluents from Onne,Nembe and Escravos polluted soils showed pH of 4.24, 5.87 and 6.3 respectively and pH>as the concentration of the effluent increased from 10-100 %. The control experiment maintained a pH of 7.6 throughout the study period. Soil conductance (Ω-1cm-1) was > in the polluted soils with peak value recorded at 100% pollution of the effluents. P, Mg, K, Ca, and Mg (mg/g) were > as the petroleumeffluents % > in the contaminated soils. TPH were consistently > in all the polluted soil. The contents of TOC and TOM correlate with the TPH with Escravos effluents exhibiting the highest levels. Peroxidase, catalase, lipase, and urease in the experimented soil were significantly > except for day 0 of 10%effluent pollution, enzyme activity in the contaminated soil samples were consistently higher than that of the control tests. Pseudomonas and Klebsiella sp. strains were found to be persistent in the polluted soil, according to analyses of soil organisms. In all the polluted soil, the peak heterotrophic counts (CFU/g) of the organisms were observed on day 0 of 10% effluent pollution, and these counts gradually declined with time. Strains of Bacillus, Pseudomonas, Streptomyces and Klebsiella sp. showed the highest emulsification index at drop collapse and oil spread plate assay. Emulsification studies by crude cultures of the isolates from the contaminated soil showed that Klebsiella sp. gave the best emulsification activitywith 51.2, 56 and 48.9% emulsification index using coconut oil, kerosene, and crude oil respectively. Pseudomonas aeruginosa with accession code of NR_075116.1; Bacillus substilis with accession code of MN421487; Klebsiella aerogene with accession code of NR_102493.2 and Streptomyces roseiscleroticuswith accession number of NR_112381. FTIR spectrum showed bands of amide I band is split intothreecomponents at 2527.1, 2206.6 cm− 1 and 1785.4 cm− 1 while amide II band is observed at 14481.4cm−1. The CH deformation of the β-glycosidic bond is centered at 853.6 cm− 1 and 711.9 cm− 1 . The current study has demonstrated the existence of several recalcitrants in petroleum effluents from these jettysiteson agricultural soils in Nigeria and this gives further credence to the importance of treating petroleum effluents before discharge.
Description
This thesis is for the award of Master of Science (MSc.) in Environmental Microbiology
Keywords
Physicochemical, petroleum effluents, quality marker enzymes, bacteria population, emulsification index, Department of Microbiology
Citation
Ani, E. A. (2023). Impact of petroleum effluent from jetty sites on physicochemical properties and microbial profile of an agricuItural soil (Unpublished Master's Thesis). Federal University of Technology, Owerri, Nigeria