Studies on anti-tumour activities of leaf extract of Diodia sarmentosa on albino rats
| dc.contributor.author | Okoroafor, Chinedu Henry | |
| dc.date.accessioned | 2026-03-21T17:04:09Z | |
| dc.date.available | 2026-03-21T17:04:09Z | |
| dc.date.issued | 2019-12 | |
| dc.description | This thesis is for the award of Master of Science (M.Sc.) in Biotechnology | |
| dc.description.abstract | This research aimed at studying the biochemical, antioxidant and anti-tumorigenic potentials of the ethanol extract of Diodia sarmentosa on Uterine Leiomyoma, Benign Prostatic Hyperplasia and Hepatocellular carcinoma in adult abino wistar rats. Uterine leiomyoma was induced by daily oral administration of 200 mg/kg body weight of monosodium glutamate (MSG) for 30 days. Benign Prostatic Hyperplasia was induced by daily subcutaneous injection of 10 mg/kg body weight of testosterone propionate for 28 days and Hepatocellular carcinoma was induced by oral administration of 20 mg/kg body weight of diethyl nitrosamine (DEN) dissolved in normal saline (0.9%) and given 5 times a week for 6 weeks. Sixty three (63) adult albino rats were used for this study and they were divided into nine groups, three groups for each of the tumours and classified as normal control (NC), positive control (PC), and the treated group (TG). In vitro studies such as 2,2-diphenyl-1- picrylhydrazyl radical scavenging method (DPPH0 ), Thiobabituric acid assay (TBARS) and Total Antioxidant Capacity (TAC) were done on the plant‘s leaves. The ethanol and aqueous extracts exhibited significant inhibition in DPPH free radical formation with IC50 values of 10.994 μg/ml and 10.121 μg/ml respectively compared to the ascorbic acid standard (IC50 value = 17.916 μg/ml). It has been reported that a lower IC50 value indicates a better antioxidant activity. The aqueous extract exhibited more inhibitory effect on TBARS with IC50 values of 2.657 μg/ml while the ethanol had an IC50 value of 8.53 μg/ml compared to BHT standard (IC50 = 2.142 μg/ml). For the total antioxidant capacity assay, the aqueousextract had higher ascorbic acid equivalent values compared to the ethanol extract. However, the two solvent extracts showed antioxidant capacity. In vivo studies were also carried out by assessing some biochemical and antioxidant parameters as well as histopathological findings. The results of the study showed an impaired antioxidant system in the positive control (untreated group) of the three study groups. Some biochemical parameters were also altered in the positive control of the three study groups mostly revealing a relationship between the various tumours and renal impairment/kidney damage. Treatment with the ethanol extract of Diodia sarmentosa significantly (P<0.05) improved the altered biochemical parameters and also significantly (p<0.05) increased the serum antioxidant levels. These findings were confirmed with a histopathology result which revealed the extent of cancer in the affected tissues and the efficacy of the ethanol extract of Diodia sarmentosa in ameliorating the damage. Conclusively, from the study, it was discovered that the ethanol extract of Diodia sarmentosa (Sw) leaves has biochemical, antioxidant and anti-tumorigenic potentials and therefore may be useful in the treatment of tumours. | |
| dc.identifier.citation | Okoroafor, C. H. (2019). Studies on anti-tumour activities of leaf extract of Diodia sarmentosa on albino rats [Unpublished Master's Thesis]. Federal University of Technology, Owerri, Nigeria | |
| dc.identifier.uri | https://repository.futo.edu.ng/handle/20.500.14562/2440 | |
| dc.language.iso | en | |
| dc.publisher | Federal University of Technology, Owerri | |
| dc.rights | Attribution-NonCommercial-ShareAlike 4.0 International | en |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
| dc.subject | Diodia sarmentosa | |
| dc.subject | biochemical | |
| dc.subject | anti-tumour | |
| dc.subject | anti-oxidant | |
| dc.subject | cancer | |
| dc.subject | Department of Biotechnology | |
| dc.title | Studies on anti-tumour activities of leaf extract of Diodia sarmentosa on albino rats | |
| dc.type | Master’s Thesis |