Bioavailability and genotoxicity of polycyclic aromatic hydrocarbons on two edible vegetables(Amaranthus hybridus and Telfiaria occidentalis)
Date
2023-11
Authors
Journal Title
Journal ISSN
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Publisher
Federal University of Technology,Owerri
Abstract
Bioavailability and genotoxic effect of polycyclic aromatic hydrocarbons (PAHs) were investigated in two edible vegetables (Amaranthus hybridus and Telfairia occidentalis) using; physicochemical properties, biotolerence of the vegetables to PAH, molecular characterization, and health risk assessment of consumption of exposed vegetables. The study assessed PAH content in different tropical urban environment using soxhlet extraction flame ionization detection (SEGC-FID) method, physicochemical properties of soil, biotolerance of the two vegetables exposed to different concentrations of spent engine oil, benzo(a)pyrene (B[a]P), benzo(k)fluoranthene (B[k]F) and benzo(ghi)perylene (B[ghi]P) in plant species, and health risk associated with the consumption of contaminated vegetables. The health risk assessment was done using health assessment models such as screening value (SV), incremental lifetime cancer risk (ILCR) and margin of exposure (MOE). Molecular characterization of exposed plant species was carried out to determine the level of toxicity on the DNA, through DNA band breaks. This was carried out with three inter-simple sequence repeat (ISSR) primers (UBC 811, UBC 827, UBC 808). SE-GCFID method was used for the separation and identification of PAH. The results showed that a total of fourteen PAHs were detected in the different tropical urban environment which includes:
acenaphthylene, phenanthrene, 1-2 benzanthene, acenaphthalene, benzo(k)fluoranthene, benzo(a)pyrene, benzo(b)fluoranthene, flourene, naphthalene, dibenzo(a,h)anthracene, anthracene, benzo(ghi)perylene, fluoranthrene and pyrene. The soil physical properties showed that the moisture content and bulk density where higher in the polluted soil, while porosity was higher in the unpolluted soil. However, the soil textural class remains sandy. In soil chemical properties; soil pH was low, effective cation exchange capacity, exchangeable base, exchangeable acidity and organic carbon were higher in the polluted soil samples. The biotolerance of the two vegetables exposed and unexposed to the pollutants showed low growth performance in the polluted plants as concentration increases. However, there was a significant difference (P<0.05) between the unexposed and the exposed plants using analysis of variance Dunnet multiple comparison. The result of the genomic effect of these pollutant revealed alteration at genetic level through DNA insertion deletion and changes in band intensity. The three primers showed polymorphism level of 65%, 58% and 18% . The health risk assessment from dietary consumption of PAH contaminated vegetables showed low health risk concern in SV, ILCR and MOE. However, prolong exposure to these pollutants can affect humans as it possesses a high potential to bioaccumulate through the food chain. The study was able to suggest the presence of PAH component in the different tropical urban environs of Owerri. Also the impact of PAH on growth performance of the two plants species showed that it is concentration dependent which was reflected in the DNA of plants species as polymorphism occurred showing variations in DNA.
Description
Master of science thesis on "Bioavailability and genotoxicity of polycyclic aromatic hydrocarbons"
Keywords
Polycyclic aromatic hydrocarbons, biotolerance bioaccumulation, molecular characterization, polymorphism, telfairia occidentalis, amaranthus hybridus., Biotechnology
Citation
Ukachukwu, C . O . (2023)Bioavailability and genotoxicty of polycyclic aromatic hydrocarbons on two edible vegetables(Amaranthus hybridus and Telfiaria occidentalis) (Unpublished Master's Thesis), Federal University of Technology, Owerri