Genomic associations between transforming growtt factor β (TGFβ3) gene polymorphism with growth performance of Nigerian improved and exotic chicken
| dc.contributor.author | Chukwuka, Nnaemeka Joseph | |
| dc.date.accessioned | 2025-06-04T11:29:10Z | |
| dc.date.available | 2025-06-04T11:29:10Z | |
| dc.date.issued | 2024 | |
| dc.description | A Master's thesis on n animal breeding and genetics | |
| dc.description.abstract | This study focused on analyzing the TGF-β3 gene polymorphism and its association with important growth and morphological traits in chickens. A total of 150 chickens from three different breeds, namely Noiler®(50), FUNAABAlpha® (FA - 50), and Sasso® (50), were included in the study. These chickens were reared from day-old chicks until they reached 20 weeks of age. Throughout this period, growth and morphological traits such as body weight, height at withers, body length, breast girth, and wing length were measured at ages 4, 8, 12, 16, and 20 weeks. Significant differences (P<0.05) were obtained on growth performance index (body weight, height at withers, body length, breast girth and wing length) with the Sasso as the best performed breed, then the Funaab Alpha and the Noiler respectively. This suggests that Sasso is good for meat production. Genomic DNA was extracted from the chickens, and polymerase chain reaction (PCR) was performed using specific primers targeting the TGFβ gene. The resulting PCR amplicons were sequenced in both forward and reverse directions to obtain accurate genetic information. Subsequently, these quences were carefully analyzed, and single nucleotide polymorphisms (SNPs) within the TGF-β3 gene were identified. These SNPs were then correlated with the recorded performance data of the chickens. Among the SNPs discovered, two variants stood out: SNP1 (A>C) and SNP2 (A>T). Notably, the CC and TT genotypes exhibited recessive characteristics. To assess the genetic diversity within the TGF-β3 gene across the three chicken breeds, haplotype and nucleotide analyses were conducted. Results revealed that Noiler and FA breeds exhibited a higher number of sequences and haplotypes (5), while Sasso breed had 4. Furthermore, the association analysis between the identified SNPs and the measured growth traits revealed interesting findings. SNP1 showed a significant association(P<0.05) with body weight and height at withers. Notably, Sasso and FA breeds demonstrated a stronger association with these traits compared to the Noiler breed. Conversely, SNP2 did not exhibit any significant associations with the measured body traits. Overall, the findings of this study suggest that the TGF-β3 gene may play a crucial role as a candidate gene, significantly influencing the body composition traits of chickens. | |
| dc.identifier.citation | Chukwuka, N. J.(2024). Genomic associations between transforming growtt factor & (TG&3) gene polymorphism with growth performance of Nigerian improved and exotic chicken (Unpublished Master's Thesis). Federal University of Technology, Owerri, Nigeria | |
| dc.identifier.uri | https://repository.futo.edu.ng/handle/20.500.14562/1981 | |
| dc.language.iso | en | |
| dc.publisher | Federal University of Technology, Owerri | |
| dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | en |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject | PCR-RFLP | |
| dc.subject | transforming growth factor-β (TGFβ) | |
| dc.subject | gel electrophoresis | |
| dc.subject | SNP | |
| dc.subject | body composition | |
| dc.subject | Department of Animal Science and Technology | |
| dc.title | Genomic associations between transforming growtt factor β (TGFβ3) gene polymorphism with growth performance of Nigerian improved and exotic chicken | |
| dc.type | Master’s Thesis |