Toxicity and oxidative effects of pesticide mixture on liver and gonads of male albino rats
| dc.contributor.author | Chukwudi, Peter | |
| dc.date.accessioned | 2026-03-18T09:55:36Z | |
| dc.date.available | 2026-03-18T09:55:36Z | |
| dc.date.issued | 2019-12 | |
| dc.description | This thesis is for the award of Master of Science (MSc.) degree in Biotechnology | |
| dc.description.abstract | Commercial mixtures of organophosphates and pyrethroids have become very popular in the insecticide markets in developing nations and have resulted in an elevation in the prevalence of mixed toxicity. The present research is aimed at evaluating the toxic effects of commercial preparation of a pesticide mixture, MagicForce, which contains Dimethoate (DM) and Lambdacyhalothrin (LC) in the ratio of 20:1. For this purpose, forty (40) adult male albino rats were divided into four (4) equal groups. Dimethoate group (DM) received: (10.7mg/kg b.w/orally/daily), Lambda-cyhalothrin group (LC) received: (3.9mg/kg b.w/orally/daily), MagicForce group (MF) received: (1.2mg/kg/b.w/orally/daily) while Control group (C) received only food and water daily. The general health of the rats was monitored daily for toxicity signs and mortality while taking the weekly body weights. At the end of the study (28 days), the rats were sacrificed. The liver and gonads were excised and weighed, the tissue homogenates prepared for biochemical analysis together with the blood samples. The estimation of the cell death marker Lactate dehydrogenase (LDH), Liver function enzymes (AST, ALT and ALP), Total Serum Protein, Total Albumin, Total Bilirubin were carried out. Oxidative markers malondialdehyde (MDA), catalase (CAT), glutathione (GSH), glutathione-s-transferase (GST) and glutathione peroxidase (GPx) were determined. Heamatological parameters (WBC, RBC, HGB and MCHC) were also examined. The reproductive status was determined by analyzing the testosterone levels, sperm motility, sperm count and sperm viability. The results revealed that nephrotic cell death occurred most in the LC treated group (73.83µ/L, 352.53µ/L, 334.53µ/L) than in the DM and MF. Liver function enzymes (AST, ALT and ALP) activities were significantly elevated in LC-treated group (138.13 µ/L, 44.73 µ/L and 29.90 µ/L) respectively, than observed in the other treatment groups. The Total Protein Profile level visibly decreased mostly in the LC exposed group (58.12µ/L, 17.19µ/L) compared to the other treatment groups. The oxidative stress and hematological studies showed that the LC-treated group was most adversely affected than the DM and Magicforce. There was no much significant impact on the reproductive status of the animals by the other pesticides except on the testosterone levels which was affected by the LC. It can be concluded that the LC, singly had more adverse effect on the overall parameters measured than the Dimethoate (DM) and in their combined commercial state (Magicforce). This suggests an antagonistic interaction in the mixture. | |
| dc.identifier.citation | Chukwudi, P. (2019). Toxicity and oxidative effects of pesticide mixture on liver and gonads of male albino rats [Unpublished Master's Thesis]. Federal University of Technology, Owerri, Nigeria | |
| dc.identifier.uri | https://repository.futo.edu.ng/handle/20.500.14562/2415 | |
| dc.language.iso | en | |
| dc.publisher | Federal University of Technology, Owerri | |
| dc.rights | Attribution-NonCommercial-ShareAlike 4.0 International | en |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
| dc.subject | Antagonistic | |
| dc.subject | dimethoate | |
| dc.subject | lambda-cyhalothrin | |
| dc.subject | magicforce | |
| dc.subject | organophosphate | |
| dc.subject | Department of Biotechnology | |
| dc.subject | pyrethroids | |
| dc.title | Toxicity and oxidative effects of pesticide mixture on liver and gonads of male albino rats | |
| dc.type | Master’s Thesis |