Prevalence of antibiotic resistant enterobacteria in selected public health care centres in Imo State
| dc.contributor.author | Ibe, Emmanuel | |
| dc.date.accessioned | 2025-06-05T11:37:40Z | |
| dc.date.available | 2025-06-05T11:37:40Z | |
| dc.date.issued | 2023-11 | |
| dc.description | This thesis is for the award of Master of science (MSc.) in Biotechnology | |
| dc.description.abstract | The greatest threat on the utilization of antibiotic medications in the treatment of bacterial nosocomial infection is the emergence and distribution of resistance in the pathogen. Most of the prevalent nosocomial bacteria have been reported to exhibit multidrug resistance which is postulated to be either acquired or natural. The present study was targeted at investigating the prevalence of antibiotic resistant Enterobacteria from some public healthcare centres in Imo State. Using randomized complete block sample design, four public healthcare centres [Imo State University Teaching Hospital (ISUTH) Orlu, Federal Medical Centre Owerri (FMC) Owerri municipal, Aboh-Mbaise General Hospital (AMGH) Mbaise, and Imo State Specialist Hospital (ISSH) Umuguma] were selected at random. Swab samples were collected, isolated, and purified using standard procedures. Kirby-Bauer disc diffusion method was employed to determine the antibiotic sensitivity of the isolates. The resistant genes were determined using forward and reverse primers specific for the following resistant genes blaTEM, qnrA, and aac(3)-1. The result showed 64.17 % growth of Enterobacteria. The highest Enterobacteria isolate was in materials from ISUTH (20.67±1.45), while the materials from FMC showed the lowest Enterobacteria isolates (18.00±1.15). Escherichia coli was the most abundant Enterobacteria (29.00 %) and Shigella spp. was the least abundant Enterobacteria (19.05 %). The isolates showed multiple resistance to some of the antibiotics tested (which included Pefloxacin, Ciproflox, Tarivid, and Augmentin). All the isolates were susceptible to Gentamicin, while area of technical uncertainty was noticed for Septrin, Cefalexin, and Streptomycin treatment on some of the isolates. The molecular characterization showed the presence of the three genes (blaTEM, qnrA, and aac(3)-1) investigated. The blaTEM gene-band was the most prevalent (80.0 %) while the aac(3)-1 gene-band was the lowest prevalent resistant gene (60.0 %). These resistant genes were most abundant in Klebsiella pneumoniae (93.33 % of the isolates) and least abundant in Shigella sp. (33.34 % of the isolates). It was concluded that the genes: blaTEM, qnrA, and aac(3)-1 were present in Enterobacteria species (Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli, and Shigella sp.) isolated from public healthcare centres in Imo State. Antibiotics stewardship should be practiced in healthcare settings. The application of antibiotic on surfaces is recommended when cleaning invasive devices and certain types of hospital equipment. | |
| dc.identifier.citation | Ibe, E. (2023). Prevalence of antibiotic resistant enterobacteria in selected public health care centres in Imo State (Unpublished Master's Thesis). Federal University of Technology, Owerri, Nigeria | |
| dc.identifier.uri | https://repository.futo.edu.ng/handle/20.500.14562/1993 | |
| dc.language.iso | en | |
| dc.publisher | Federal University of Technology, Owerri | |
| dc.rights | Attribution-NonCommercial-ShareAlike 4.0 International | en |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
| dc.subject | Antibiotics | |
| dc.subject | enterobacteria | |
| dc.subject | sensitivity | |
| dc.subject | prevalence | |
| dc.subject | resistant genes | |
| dc.subject | Department of Biotechnology | |
| dc.title | Prevalence of antibiotic resistant enterobacteria in selected public health care centres in Imo State | |
| dc.type | Master’s Thesis |