Potential production of lipases by pseudomonas and staphylococcus species isolated from palm oil contaminated tropical soil
Date
2012-05-20
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
U. P.
Abstract
This study reports on the potential production of lipases by Pseudomonas and Staphylococcus species isolated from palm oil contaminated tropical soil. The lipase activities of Pseudomonas species and Staphylococcus species at 37oC and pH 7 were evaluated. Both organisms grew well and produced lipases at the prevailing assay conditions. The lipase activity by Pseudomonas species was significantly higher than that of Staphylococcus species at P< 0.05. Effect of pH on the enzyme activities of the isolates showed that lipase production varied according to the pH of the assay medium. This shows that pH is a determinant factor in lipase production. There were increases in lipase activities with increase in pH up to optimum pH of 7 in both organisms. The lipase activities decrease progressively after this optimum pH till the last pH of exposure (pH 10). At pH 7 and 8, the two organisms had equal lipase activities (0.64 and 0.63 U/ml/min, respectively), after which, the lipase activity of Pseudomonasspecies reduced gradually with increase in pH unlike Staphylococcus species whose lipase activity abruptly decreased with increase in pH. Lipase activities in both organisms were affected differently by varying temperature changes. In Pseudomonas species, there was a steady increase in lipase activity with increase in temperature up to the optimum at temperature of 30 oC (0.67 U/ml/min). The activity decreased slightly at temperature of 35 oC but effect of temperature against the activity was more pronounced at the temperatures of 40 and 45 oC. The lipase activity of Staphylococcus species was also affected by temperature changes. There was a steady increase in the activity up to the optimum at temperature of 35 oC. The activity started decreasing steady with further increase in temperature. In conclusion, the study showed that Pseudomonas and Staphylococcus species isolated from palm oil contaminated tropical soil has a great potential for the production of lipases.
Description
This research was conducted in collaboration with a staff of FUTO
Keywords
Potential, production, lipases, speudomonas species, staphylococcus species, palm oil, tropical soil, Department of Microbiology
Citation
Odu, N. N. & Akujobi, C. O. (2012). Potential production of lipases by pseudomonas and staphylococcus species isolated from palm oil contaminated tropical soil. Cancer Biology 2(2), 45 - 49