School of Biological Sciences
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Browsing School of Biological Sciences by Subject "assessment"
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Item Open Access Bacterialogical and parasitological assessment of fresh meat marketed in Owerri, Imo State, Nigeria(U. P., 2015) Mgbemena, I. C.; Ebe, T.; Nnadozie, A. I.; Iloanya, U. C.The bacteriological and parasitological assessment of some fresh meat marketed in Owerri, South – eastern, Nigeria was carried out using standard bacteriological and parasitological methods. The meat samples used for the study were flesh, towel, intestine and liver from goat, pork and chicken. There was no fungal and Salmonella - Shigella counts in all the samples. The presence of Staphylococcus count was obtained in fresh goat meat and the viable bacterial counts ranged from 5.0× 105cfi/ml to 8.0× 106 cfu/ml while the total coliform counts ranged from 1.0× 105(cfu/ml to 4.0×106cfu/ml. The bacterial isolates obtained were: Staphylococcus aereus, Micrococcus species, Salmonella species, Shigella species, Corynebacterium species, Pseudomonas aureginosa, Bacillus species and Escherichia coli with Micrococcus species showing the highest occurrence. Among the parasites identified, Taenia spp had the highest occurrence. These organisms are known to be of public health importance and therefore, there is need for proper hygienic practices to be observed by the butchers in addition to beefing up the activities of Consumers Protection Council (CPC) to ensure the safety of meat available for public consumption.Item Open Access Bacteriological assessment of packaged fruit juices consumed in some parts of Anambra, Nigeria(Academic and Scientific Research Institute Publication, 2010) Okereke, J. N.; Onyeneto, T. C.Bacteriological assessment of eighteen samples of different fruit juices consumed in parts of Anambra State was carried out using standard microbiological procedures. Both total heterotrophic bacteria count and total coliform counts were determined using 0.1ml of each seria11y diluted sample on nutrient and MacConkey agar plates respectively. Total elerolrophic bacteria count was of the order x I 04 cfu/ml while total coliform count was of the order x103 cfu/ml. Frequently isolated microorganisms were Laciobacilius spp, Bacillus spp, SJaphylococcus spp, Psuedomona.s spp and Micrococcus spp. Virtually all the sampled fruits juices had some degree of bacterial contaminationItem Open Access Physico-chemical and bacteriological assessement of Oyibo River in Ehime Mbano, Imo State(U. P., 2015) Okereke, J. N.; Okanume, J. N.; Okereke, C. B. N.The physico-chemical and bacteriological assessement of Oyibo river, Ehime Mbano, was carried out between 2011 and 2012. Considering the communities that make use of this river for different purposes, water samples were collected from four different sampling points along the river and subjected to physico-chemical and bacteriological analyses. Pour plate method was adopted to determine total heterotrophic bacteria, and membrane filtration technique, for total coliforms and E. coli counts. Standard procedures for water analysis by APHA were adopted for the determination of physical and chemical parameters. Atomic Absorption Spectrophptometer (AAS) was used to analyze for heavy metals. The average values of total heterotrophic bacteria were 5.8 x 103 cfu/ml and 1.51 x 103 cfu/ml for rainy and dry seasons respectively. Average values for total coliforms and E. coli counts were 33.75 cfu/100ml and 6 cfu/100ml for rainy season, and 17.38 cfu/100ml and 4 cfu/100ml for dry season. Bacterial isolates included E. coli, Salmonella spp, Lactobacilli spp, Klebsiella spp, Staphylococcus spp, Proteus spp, and Pseudomonas spp. In all the water samples, the pH values ranged from 6.04– 6.52. Average values of total hardness for rainy and dry seasons were 5.90mg/l and 5. 43mg/l respectively. The concentrations of nitrate, phosphate, chloride, and sulphate ranged from 0.00 16.40mg/l; 0.01– 1.24mg/l; 0.13– 0.35mg/l; 0.00– 15.95mg/l respectively. Average COD, BOD and DO values were 5.63mg/l, 2.07mg/l, and 3.17mg/l in dry season, and 9.24mg/l, 1.77mg/l, and 4.26mg/l in rainy season. The values of heavy metals during the rains were within the range of 0.0008ppm– 1.5605ppm, but 0.0001ppm– 0.0339ppm, in dry season. There were no significant differences in some bacteriological and chemical properties with season, but the physical parameters such as depth, flow rate, pH, and turbidity were, at p ˂ 0.05. The study revealed that Oyibo River is not safe for drinking (inasmuch as it serves as source of drinking water for some communities), unless subjected to appropriate treatment.