Bioassay-guided isolation, purification and charaterization of antimicrobial compound from acidic metabolite of piper umbellatum seed extract

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dc.contributor.authorEjele, A. E.
dc.contributor.authorAkalezi, C. I.
dc.contributor.authorIwu, I. C.
dc.contributor.authorUkiwe, L. N.
dc.contributor.authorEnenebaku, C. K.
dc.contributor.authorNgwu, S. U.
dc.date.accessioned2024-12-02T13:03:17Z
dc.date.available2024-12-02T13:03:17Z
dc.date.issued2014
dc.descriptionThis article contains tables and figure.
dc.description.abstractThe antimicrobial potential of ethanol extract of Piper umbellatum seeds and its acidic, basic and neutral metabolites were evaluated using the disc diffusion method. The results showed that the acidic and neutral metabolites exhibited greater antibiotic activities against the tested microorganisms; Coliform bacilli, Salmonella typhi and Staphylococcus aureus and gave inhibition zone diameters greater than 28 mm against both C. bacilli and S. aureus. These results were interpreted in terms of differences in phytochemical composition of these metabolites and indicated that an effective anti-Staphylococcal drug could be developed from P. umbellatum extract for chemotherapy of diseases caused by S. aureus, which is known all over the world to develop resistance to most potent antibiotic drugs. Chromatographic purification of the acidic metabolite gave five fractions whose antimicrobial potential against some pathogens was evaluated and compared with Amoxil (a positive standard antimicrobial drug). The results showed that all the chromatographic fractions possessed inhibitory activities against the tested microbes. Spectroscopic analysis of IR, 1H– and 13C–NMR data suggested that the active principle in acidic metabolite of P. umbellatum could be Naringin, a naturally occurring polyphenol and antibiotic drug.
dc.identifier.citationEjele, A. E., Akalezi, C. I., Iwu, I. C., Ukiwe, L. N., Enenebaku, C. K. & Ngwu, S. U. ( 2014). Bioassay-guided isolation, purification and charaterization of antimicrobial compound from acidic metabolite of piper umbellatum seed extract. International Journal of Chemistry, 6(1), 61 - 70
dc.identifier.doi10.5539/ijc.v6n1p61
dc.identifier.issne-1916-9701
dc.identifier.issnp-1916-9698
dc.identifier.urihttp://dx.doi.org/10.5539/ijc.v6n1p61
dc.identifier.urihttps://repository.futo.edu.ng/handle/20.500.14562/1567
dc.language.isoen
dc.publisherCanadian Center of Science and Education
dc.relation.ispartofseriesISSN
dc.relation.ispartofseriese- 1916-9701
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.subjectIsolation
dc.subjectcharacterization
dc.subjectactive principle
dc.subjectacidic metabolite
dc.subjectPiper umbellatum
dc.subjectDepartment of Chemistry
dc.titleBioassay-guided isolation, purification and charaterization of antimicrobial compound from acidic metabolite of piper umbellatum seed extract
dc.typeArticle

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