Browsing by Author "Okoro, O. A."

Now showing 1 - 1 of 1
  • Thumbnail Image
    ItemOpen Access
    Determination of the binding characteristics of dicoumarol to bovine serum abumin by UV/visible spectroscopic method
    (U. P., 2020) Okoro, O. A.; Onwumere, F. C.; Enebeaku, C. K.; Akalezi, C. O.
    8-Anilinonaphthalene-1-Sulfonic acid (ANS) was used as a probe to study the binding characteristics of Dicoumarol to Bovine Serum Albumin (BSA). The absorbance of ANS-BSA complex was decreased by the binding of Dicoumarol. This suggests that there is competition between Dicoumarol and ANS for the binding sites in the BSA. It means that ANS and Dicoumarol shared some of the binding sites on BSA. From the scatchard plot for the binding of Dicoumarol to BSA it is seen that Dicoumarol has three high affinity binding sites with association constants Kl=14.5245 x 106,10.6281x106,11.6811x106 respectively, and three secondary binding sites with association constants Kl =14.0084 x 10615.8816 x 106 and 19.2058x106 respectively and numerous low affinity binding sites.