Browsing by Author "Ogbulie, J. N."

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    Antibiotic-resistant Escherichia coli in a government piggery farm in Owerri, Nigeria
    (International Formulae Group, 2008) Akujobi, C. O.; Ogbulie, J. N.; Umeh, S. I.; Abanno, N. U.
    The acquisition of antibiotic-resistant commensal Escherichia coli was examined in a cohort of newborn pigs. Faecal samples were collected weekly from young pigs over a 2 month period and screened for E. coli resistant to ampicillin, nalidixic acid, gentamicin and ciprofloxacin at concentrations of 16, 8, 8 and 8mg/l, respectively. E. coli viable counts were also performed on the samples. All the pigs acquired gentamicin-nalidixic acid- and ampicillin-resistant E. coli, while 80% acquired ciprofloxacin-resistant E. coli during the study. Sixty-five per cent of samples were resistant to at least one of the four antibiotics. The prevalence of the antibiotics declined significantly with age and time (p<0.05). Total counts and antibiotic resistant counts declined with age (p<0.05), with the rate of decline in antibiotic resistant counts being greater than that for total counts (p<0.05). The cohort pigs rapidly acquired antibiotic-resistant bacteria shortly after birth and the carriage of resistant bacteria was associated with age.
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    Asymptomatic bacteriuria in pregnant women at the outpatient clinic of some governmental hospitals in Imo State, Nigeria
    (International Formulae Group, 2009) Akujobi, C. O.; Ogbulie, J. N.; Umeh, S. I.; Abanno, N. U.; Nwachukwu, I. N.
    Asymptomatic bacteriuria among pregnant women in Imo State, Nigeria was studied. All pregnant women attending antenatal at Federal Medical Center, Owerri, General Hospital, Okigwe, and Imo State University Teaching Hospital, Orlu, between January and April 2006 and who agreed to enter the study were clinically evaluated to exclude signs of urinary tract infection. Samples of 10-15 ml urine were examined for bacteria, pus cells and parasitic ova. The samples were further cultured on cysteine lactose electrolyte deficient agar and colony counts yielding bacterial growth of 105 or more of pure isolates were deemed significant. Of the 630 pregnant women, 111 had significant bacteriuria giving a prevalence rate of 17.6%. The highest age specific prevalence was found in the 31-40 year-olds and the lowest in the 41-50 year-olds. Socioeconomic status of the respondents had effect on the prevalence of bacteriuria and also, there was an increase in the prevalence with increase in age of gestation. The dominant isolates were Staphylococcus species and Klebsiella species with most of the isolates obtained in respondents from Owerri and Okigwe study areas.
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    Effect of different organic nutrient supplements on the total heterotrophic counts and diesel oil degradation in diesel polluted soil
    (International Formulae Group, 2009-12) Akujobi, C. O.; Njoku, H. O.; Abanno, N.; Okorondu, S. I.; Ogbulie, J. N.
    The effect of graded quantities of different organic nutrient supplements on the total heterotrophic counts and diesel oil degradation in diesel-polluted soil was studied. Soil samples were mixed with different volumes of diesel to obtain heavy and moderate pollution, which was subsequently amended with different quantities of organic nutrient supplements. Total heterotrophic counts were determined at two weeks intervals for 16 weeks and the oil and grease content were determined at the beginning and at the end of the study. The result showed a general increase in microbial count with increase in the quantities of supplement. Poultry waste-amended samples had the highest count in all the samples followed by pig waste and cow dung-amended samples. Diesel oil degradation followed the same order with the highest percentage oil degradation obtained in poultry waste-amended samples. It therefore, showed that poultry waste is the best organic waste to be applied in a bio degradation process.
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    Occurrence of extended-spectrum β lactamases in Escherichia coli isolated from piggery farms in Imo State, Nigeria
    (Springer Science+Business Media, 2008) Akujobi, C. O.; Ogbulie, J. N.; Alisi, C. S.
    A total of 600 Escherichia coli isolates recovered from pig wastes from three Senatorial Zones of Imo State, Nigeria, were tested for production of extended spectrum b-lactamases (ESBLs) by the double-disk (DD) potentiation method. Of the numbers of isolates made, 190(32%) were positive for the ESBLs production. Results of testing 190 positive isolates for ESBL production by several recommended methods were as follows (percentage detection in parentheses): DD method with aztreonam (91), ceftazidime (82), ceftriaxone (84), or cefpodoxime (94); broth microdilution method with ceftazidime (77) or cefotaxime (96) alone or in combination with clavulanate; and the standard disk diffusion method with new breakpoints and standard concentrations of aztreonam (69), ceftazidime (81), ceftriaxone (77), or cefpodoxime (98) or a novel concentration (5 lg) of ceftazidime (84). These data indi cate that ESBLs occur at a relatively high incidence in our piggery farms and that the standard disks diffusion method with cefpodoxime and the DD method with several b-lactams are practical and cost-effective methods for detecting ESBL-producing isolates of E. coli.