Browsing by Author "Nwacha, R."

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    Optimization of bioremediation of cheese whey with the activity of klebsiella pneumonia using response surface methodology
    (U. P., 2013) Azeez, T. O.; Onukwuli, O. D.; Araromi, D. O.; Arinkoola, A. O.; Salam, K. K.; Iwuji, S. C.; Ejeta, K. O.; Dawodu, B. F.; Ayinde, K. A.; Nwacha, R.; Azeez, F. O.
    Response surface methodology was used to study the optimization of bioconversion of cheese whey to 2,3-Butanediol using Klebsiella pneumonia at room temperature. 3-Level factorial design was employed to correlate the bioremediation parameters with the biomass of K. pneumonia and concentration of 2,3-BD as response. Quadratic polynomial equation was developed to achieve optimal performance of the process. The result showed that the optimum condition for the bioremediation process in unaerated and airlifted batch reactors for concentration of cheese was 39.98g/L and 39.94g/L, and fermentation time was 95.5hours which resulted in biomass of k. pneumonia 1.4633mg/L and 3.6580mg/L, and 2,3-BD production of 10.696g/L and 17.997g/L respectively. 2,3-BD production in unaerated and airlifted batch reactors amounted to 36.88% and 62.06% respectively. The study has shown that k. pneumonia not only utilized cheese whey as a source of food and energy but optimally better for remediation of cheese whey in airlift batch reactors.