Browsing by Author "Braide, W."

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    Bioelectricity production and treatment of Abattoir wastewater in a ferricyanide catholyte H-type microbial fuel cell
    (Society for Advancement of Sciences, 2016) Egbadon, E. O.; Nweke, C. O.; Akujobi, C. O.; Braide, W.; Akaluka, C. K.; Adeleye, S. A.
    Dual-chamber Microbial Fuel Cells (MFCs) were constructed using non-reactive polyacrylic containers of 1100ml with a working volume of 1000ml. 1000ml of the abattoir wastewater was fed into the anode chamber while equal volume 100mM Potassium Ferricyanide solution was fed into the cathode chamber. An Agar-salt Bridge (2% Agar and 1% NaCl) with dimension 10cm×3cm (length and radius) served as Proton Exchange Membrane. Rod-shaped carbon electrodes of length and diameter 12 cm × 1.2 cm were used. The Open circuit voltage, current, power density and physicochemical parameters were monitored. An initial Open circuit voltage of 459 mV, Current of 0.22 mA, and Power density of 22.10mW/m2 were recorded, which increased to give maximum Open Circuit Voltages of 736 mV, Current of 0.46mA, and Power density of 66.43mW/m2.The results also shows a 56.09%, 92.31%, 56.27%, 89.92%, 73.29% and 75.46% decrease for Chemical Oxygen Demand (COD), Biochemical Oxygen Demand (BOD), Organic Carbon, Total Soluble solids (TSS), Total Dissolved Solids (TDS), Nitrate, and Nitrate-Nitrogen respectively, while a -3.58%, -3.51%, -4.21%, -228.76%, -226.07% and -226.16% increase was observed for Phosphates, Phosphorus, Orthophosphates, Ammonia, Ammonium Nitrogen and Ammonium respectively. The bacterial isolates identified were Bacillus species, Streptococcus species, Escherichia coli and Staphylococcus aureus.
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    Hydrocarbon degradation potential by soil bacteria using vapour-phase and spectrophotometric methods
    (U. P., 2020) Azuwike, C. O.; Ahumibe, N. C.; Mgbemena, I. C.; Nwanaforo, M.; Braide, W.
    Crude and refined oil contamination is inevitable is oil producing communities. The attendant effects of environmental degradation and low crop yield had remain a burden to mankind. Soils from oil polluted areas were assessed microbiologically using vapour phase and spectrophotometric methods. This study assessed bacteria with capabilities of utilizing diesel, toluene and kerosene as sole source of carbon for growth. Culturing and sub-culturing was done in nutrient agar and Bushnell Haas Agar (BHA).Micrococcus luteus, Enterococcus fecalis, Bacillus subtilis, Bacillus cereus and Staphylococcus aureus were the predominant microorganisms isolated from the samples locations. The standard plate counts for each source of sample include FA1 (2.80 × 106), FA2 (1.50 ×106), MW1 (3.80 × 106), MW2 (1.7 × 106), FS1 (1.22 × 107), and FS2 (1.30 × 103). Spectrophotometric reading showed visible growth/turbidity in tubes labeled MW1bT, MW1bK, MW1bdD, FS2bD, and FS2bT. The growth of bacteria was observed from visible increase of turbidity and was enumerate by CFU/ml. Among isolates, Bacillus subtilis exhibited relatively higher ability to grow on the three hydrocarbons. Microorganisms have metabolic capabilities to utilize the carbon compounds and degrade them to innocuous by-products. These abilities can be targeted for bioremediation purposes.