Browsing by Author "Banigo, Alma Tamunonengiofori"

Now showing 1 - 2 of 2
  • Thumbnail Image
    ItemOpen Access
    Recombinant phosphoinositide-3-kinase C2β C2 domain molecules
    (IISTE, 2017) Banigo, Alma Tamunonengiofori; Azeez, Taofik Oladimeji
    Recombinant DNA domain remains a dependant of effective use of recombinant proteins in many applications but its production remains an area of concern due to DNA vector and production path. This work is aimed at studying the generation and replication of the recombinant DNA molecule using PI3K C2β C2 domain and glutathione s-transferase (GST) which expressed in pGEX-2T vector. The molecular cloning technique was employed to generate a recombinant DNA molecule. PI3K C2β C2 domain of isoform PI3K C2β belonging to class PI3K C2 of Phosphoinositide-3-Kinase (PI3K) family was used and the effective digestion of pGEX-2T vector was studied using restricted enzymes (RE) of EcoR I and Sma I with binding partners location. The ligated product of recombinant DNA was obtained after successful doubled digestion of pGEX-2T vector. The best transformation of competent bacterial cells was obtained at molar ratio of 5:1 for RE digested vector insert DNA and vector DNA. The recombinant DNA can be employed may be used in treatment of non – communicable diseases such as cancer and diabetes.
  • Thumbnail Image
    ItemOpen Access
    The impact of mentoring programs for sustainable career development and work/life balance in Biomedical Engineering profession - Short communication
    (U. P., 2019) Banigo, Alma Tamunonengiofori; Iwuji, Samuel Chidi; Ezelote, Judith Chinelo; Azeez, Taofik Oladimeji
    The socio-economic, technological and emotional imbalance remains an issue of individual growth in career development and work-life balance. The impact of mentoring programs for sustainable career development and work and lifestyle balance in biomedical professions was examined. Career development was studied using self-assessment, career awareness, performance, skill acquisition, goal setting and work-life style balance. Career development can be achieved with pleasure if the essential routes were addressed by the parties involved in mentoring programs. Proffered solution of organizational support, social support, use of modern facilities and self-assessment were recommended as tools for mentoring programs that reduced pain, depression, friction and wear, and emotional stress of mentors and mentees. Thus, provide an outstanding strength of social, economic, technological and emotional output as well as good healthy conditions for career development and stable work-lifestyle to achieve a set goal and target in biomedical engineering profession based on action plans.