Browsing by Author "Akujobi, Campbell Onyeka"

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    Parametric optimization of synthesis of silver nanoparticles from Mangifera indica and Prunus dulcis extracts and their antibacterial activity
    (University of Oradea Publishing House, 2020) Akujobi, Campbell Onyeka; Anuforo, Henry Uzoma; Okereke, Josephat Nwabueze; Ibeh, Chinyere; Agbo, Chioma Joy
    Optimization of process parameters is crucial to the deployment of nanotechnology as a competitive source of novel materials to many fields. The present study has lent credence to the simplicity, environmental friendly, cost effective and quick potentials of using plant extracts as source of capping and stabilization agents in the synthesis of silver nanoparticles (AgNPs). Aqueous leaf extracts of Mangifera indica and Prunus dulcis were used in synthesis of silver nanoparticles. The study was designed with Box Behnken Design (Minitab® 17) to optimize temperature (25 – 35°C), pH (6 – 8) and time of reaction (6 – 24hours). Fifteen runs were obtained for each sample which determined the value of each parameter used for the synthesis. Results obtained were subjected to Response Optimizer (Minitab® 17) which predicted optimum conditions for synthesis of silver nanoparticles as 25°C at pH 8 and 10.24 hours with predicted maximum yield of 2.53 for Prunus dulcis. However, the actual yield of silver nanoparticles under these conditions was 2.64. For Mangifera indica leaf extract, the predicted optimum conditions were 31.4°C at pH of 8.0 and 9.39 hours with predicted maximum yield of 2.55. Nevertheless, the actual yield under the optimum conditions was 2.61. Results show that Prunus dulcis extract has relatively higher potential yield for silver nanoparticles than Mangifera indica extracts. UV-Vis spectrophotometer showed that the absorbance for synthesized silver nanoparticles using both plant extracts peaked between 400 – 430nm. Silver nanoparticles from both plants showed activity against Bacillus subtilis and Pseudomonas aeruginosa, though B. subtilis was more sensitive. However, silver nanoparticle from Prunus dulcis was less effective against the bacteria.