Bob Chile-Agada, Adaeze2025-02-262025-02-262021-07Bob, C. A. A. ( 2021 ). Biochemical and carcinogenic effects of monosodium glutate and soya bean in wistar rats (Unpublished Doctoral Thesis). Federal University of Technology, Owerri, Nigeriahttps://repository.futo.edu.ng/handle/20.500.14562/1654The thesis is for the award of Doctor of Philosophy Degree ( Ph.D) in Enzymology BiochemistryThis study was carried out to investigate the biochemical effects of short, medium and long term administration of monosodium glutamate (MSG) and soya beans in both male and female Wistar rats, using standard methods. LD50 was carried out using rats which led to the choice of 1000, 2000 and 3000 mg/kg b.w for low, medium, and high doses of the samples. A total of 210 (105 males and 105 females) weanly Wistar rats weighing 70 – 78g were divided equally into three groups of 70 rats each representing the various experimental durations (2, 4, and 6 months). Each of these groups containing 70 rats was further divided equally into 14 subgroups, each containing 5 rats. Group 1 and 8 were controls for female and male rats fed normal raw chow and saline. Groups 2-7 (female rats) and 9-14 (male rats) were rats administered low dose (LD), medium dose (MD) and high dose (HD) MSG and soya beans respectively. 6-o-Malonylgenistin (98.47%) and Glycitin (98.11%) were the most abundant oestrogen-like compounds in the dehulled and oil of soya beans. The glucose and insulin levels of both male and female rats were significantly altered by HDs of both MSG and soya beans after 6 months while ALT, ALP and AST levels were significantly elevated after 4 and 6 months following administration of both MSG and soya beans and were more extensive in HD soya bean fed rats. No significant changes (P> 0.05) were observed in the urea levels after 2 and 4 months soya beans respective administration to female and male rats, while HD MSG significantly elevated the creatinine levels of male and female rats after 4 and 6 months administration. Administration of soya beans and MSG for 2 and 4 months had no significant effect (P > 0.05) on the bicarbonate and chloride levels. The HDL levels were significantly reduced while LDL, TC, and TG were significantly (P < 0.05) elevated after 6months HD administration of MSG and soya beans. Soya beans administration produced no significant change in the SOD levels for female rats and catalase levels in male rats after 2 months administration whereas the MD and HD administration of MSG significantly elevated MDA levels of both male and female rats after 4 and 6 months. Two and four months administration of soya beans and MSG produced no significant changes (P > 0.05) in the LH and FSH of female rats while MD and HD of MSG and soyabean significantly decreased the testosterone and LH of male rats. The colorectal, pancreatic, and ovarian cancer markers, and prostate specific antigen levels were significantly elevated after administration of MD and HD of MSG for 2, 4, and 6 months. This study has shown that the high dose administration of monosodium glutamate and soya bean produced delecterious effects on the biochemical parameters and cancer markers on rats after six months extensive administration.enAttribution-NonCommercial-ShareAlike 4.0 InternationalBiochemical indicescancer markershistopathologymonosodium glutamateoestrogen-like substancessex hormonessoyabeanwistar ratsDepartment of BiochemistryDoctoral Thesis